RESEARCH ARTICLE


Effects of Achyranthes aspera Extracts on the Survival and Midgut Histo-architecture of Aedes aegypti L. Early IV Instars



Aarti Sharma1, Sarita Kumar2, *, Pushplata Tripathi1
1 School of Sciences, Indira Gandhi National Open University, Maidan Garhi, New Delhi 110068, India
2 Department of Zoology, Acharya Narendra Dev College, University of Delhi, Kalka Ji, India


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 932
Abstract HTML Views: 305
PDF Downloads: 176
ePub Downloads: 122
Total Views/Downloads: 1535
Unique Statistics:

Full-Text HTML Views: 450
Abstract HTML Views: 220
PDF Downloads: 130
ePub Downloads: 93
Total Views/Downloads: 893



© 2018 Sharma et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to the author at the Department of Zoology, Acharya Narendra Dev College, University of Delhi, Kalka Ji, India; Tel: +919810309912; E-mail: saritakumar@andc.du.ac.in


Abstract

Background:

Aedes aegypti L.; one of the most important insect vectors in the world; transmits several diseases of concern; Zika, yellow fever, Chikungunya, dengue and dengue haemorrhagic fever. Despite multifarious problems on humans, non-targets and environment; caused by synthetic chemical insecticides; these are still the prime and preferred control measures against dengue vector. Alternative control strategies using eco-friendly and bio-degradable plant products are being explored.

Objective:

The present study investigates the toxic potential of the hexane extract of the leaf and stem of Achyranthes aspera against Ae. aegypti.

Methods:

The larvicidal potential of extracts was evaluated against dengue larvae as per WHO protocol. Subsequent concentration and time-dependent studies assessed their effects on the larval midgut histo-architecture using microtomy techniques.

Results:

Larvicidal bioassays with A. aspera extracts revealed their appreciable larvicidal potential. Hexane extract of the leaf resulted in respective LC30, LC50 and LC90 values of 67, 83 and 140 ppm while exposure to hexane extract of the stem showed respective values of 55, 68 and 115 ppm. Extract-exposed larvae at various lethal levels exhibited significant damage, shrinkage, distortion and vacuolization of gut tissues and peritrophic membrane. The disintegration of epithelial cells and cytoplasmic organelles evidenced stomach poison potential of the extracts. The extent of toxicity and damage was concentration and time-dependent; the stem extract imparted more deleterious effects as compared to the leaf extract.

Conclusion:

Present findings suggest the utilization of A. aspera as an alternate control strategy against Ae. aegypti; though further studies against non-targets are needed to ascertain its use in the fields.

Keywords: Achyranthes aspera, Aedes aegypti, Histological architecture, Larvicidal, Midgut, Stomach poison.