RESEARCH ARTICLE


Identification of Trypanosoma Strains Isolated in Central and South America by Endoncleases Cleavage and Duplex PCR of Kinetoplast-DNA



Isabel Rodríguez-Gonzáleza, Clotilde Marína, Gregorio Pérez-Cordóna, Ramón Gutiérrez-Sánchez b, Manuel Sánchez-Moreno *, a
a Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, C/ Severo Ochoa s/n. 18071 Granada, Spain
b Departamento de Estadística e I.O, Facultad de Ciencias, Universidad de Granada, C/ Severo Ochoa s/n. 18071 Granada, Spain


© 2008 Rodríguez-González

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, C/ Severo Ochoa s/n. 18071 Granada, Spain; Tel: +34 958 242369; Fax: +34 958 243174; E-mail: msanchem@ugr.es


Abstract

In this study, we report the characterization of seven trypanosome stocks isolated in different geographical areas of Latin America and from different vector species, by analysis of kinetoplast DNA (kDNA) restriction fragmentlength polymorphism, using five different restriction endonucleases, and genomic DNA by duplex PCR assay. According to the statistical study, the stocks were grouped into three clusters: cluster 1 held the three stocks of T. cruzi used as references. Very close to these were the stocks isolated in the Peruvian Amazon (TP702, TP704 and TP706), which constituted cluster 2. This cluster also included stock TP504, although this would be a hybrid between T. cruzi and T. rangeli. Cluster 3 consisted of the trypanosomes isolated from salivary glands (TRa, TRa605, TRa606 and TM5), these four stocks being the same as T. rangeli, and the phylogenetic separation observed could be due to having been isolated T. cruzi.